monosaccharide testing solution

Monosaccharides testing solution is one of way to find out the amount of sugar in food. – Carbohydrates are an important source of food energy. Carbohydrates exist in foods in different forms: monosaccharides (single sugars), disaccharides (double sugars), and polysaccharides (many sugars).

In this project, you will test for the presence of the different forms of carbohydrates. Foods containing simple sugars will be identified and the amount of sugar in each compared. You will also look at differences between complex and simple sugars.

Learning Objective

To test for the presence of monosaccharides (simple, single molecules of sugar)—in this case, glucose and fructose.


2-quart (1-liter) cooking pot



2 tablespoons (30 ml) of each liquid food sample: soda, diet soda, honey, apple juice

1 tablespoon (15 ml), or a peasize sample, of each solid food sample: banana, sugar, onion, pasta

8 baby-food jars

distilled water

monosaccharide testing solution (see How to make monosaccharides solution)

forceps or pot holder


1. Prepare a water bath by filling the pot with 2 inches (5 cm) of water.

2. Place the pot of water on the stove and heat to boiling.

3. While the water is heating, prepare the food samples by placing each sample in a separate baby-food jar (see Figure).

figure materials of testing monosaccharides

4. Add 1 tablespoon (15 ml) of distilled water to each jar containing a solid food sample.

5. Prepare a data table such as the one shown here. Note: List all eight food samples.

data table of testing food

6. Test for the presence of monosaccharides in each food sample by:

• adding 1 tablespoon (15 ml) of testing solution to the jar.

• observing and recording the color of the solution in the jar.

• setting the jar in a pot of boiling water and heating for three minutes.

• using forceps or a pot holder to carefully remove the hot jar.

• observe and record the color of the solution in the jar again.

7. Determine the concentration of the monosaccharide present in each food sample by comparing the final color of the solution with the following color chart:

blue—zero                                        orange to red—high


green—low concentration

light to dark yellow—moderate



The author’s results indicated that the soda, honey, apple juice, and onion had the highest amount of simple sugars; the banana, a moderate amount; and the pasta, diet soda and table sugar (sucrose), no monosaccharides. Note: The results will vary depending on the brand of food tested and on the conditions and length of time the food has been stored.


The monosaccharide testing reagent contains copper II ions (Cu++). Solutions containing copper II ions are blue. Sugars such as glucose and fructose provide electrons needed to change the copper II ions to copper I ions (Cu+) and elemental copper (Cu0).

These simple sugars function as reducing agents because they provide electrons during a chemical reaction. The color of the copper testing solution changes from blue to red as the copper II ions are first changed into copper I and then elemental copper. With disaccharides or polysaccharides, the testing solution remains blue.

You can read the other articles of #nature-school in this website.